Hardikar, PhD, received MSc in Zoology (Genetics) and a PhD in Zoology from the
Research in the laboratory is focused on understanding islet biology and development of insulin-producing cells. We work with cadaveric human pancreatic islets as well as biliary duct and gallbladder-derived cells to gather information that would help us understand development of insulin-producing cells. Our previous studies using next generation sequencing of developing human pancreas using the SOLiD platform have provided insight to understanding the role of ncRNAs (specifically microRNAs) in development and differentiation of insulin-producing cells. Present research projects involve applying this information to differentiation of human pancreatic progenitor cells. In addition to these studies, other projects in the lab are focused on understanding the epigenetic modifications in insulin-producing cells in a unique model of multigeneration undernutrition. These studies involve understanding the influence of diet, micronutrients, intrautetine programming and gut microbiota in development of central adiposity, insulin resistance and type 2 diabetes.
Group leader | Diabetes and Islet-biology Group,
NHMRC Clinical Trials Centre, Faculty of Medicine, The University of Sydney
Camperdown, NSW 2050, Australia
Off: +61 (02) 9562 5071
(For area map, click here)
Centre of Excellence in Epigenetics, Indian Institute of Science Education and Research, 900,
NCL Innovation Park, Dr Homi Bhabha
Pune, Maharashtra 411008, India
Centre of Excellence in Epigenetics,
Indian Institute of Science Education and Research, 900,
NCL Innovation Park, Dr Homi Bhabha
Stem Cells and Diabetes Section,
National Center for Cell Science, Ganeshkhind Road,
Pune 411007, India
· Joglekar MV, Trivedi P, Kay TW, Hawthorne WJ, O’Connell PJ, Jenkins AJ, Hardikar AA and Thomas HE (2016) Human islet cells are killed by BID-independent mechanisms in response to FAS ligand. (in press)
· Joglekar MV, JanuszewskiAS, Jenkins AJ and Hardikar AA (2016) Circulating MicroRNA Biomarkers of Diabetic Retinopathy. 65(1):22-4. doi: 10.2337/dbi15-0028.
· Jenkins AJ, Joglekar MV, Hardikar AA, Keech AC, O'Neal DN, Januszewski AS (2015) Biomarkers in Diabetic Retinopathy. Rev Diabet Stud.;12(1-2):159-95. doi: 10.1900/RDS.2015.12.159.
· Kim KW, Ho A, Alshabee-Akil A, Hardikar AA, Kay TW, Rawlinson WD, Craig ME (2015) Coxsackievirus B5 Infection Induces Dysregulation of MicroRNAs Predicted to Target Known Type 1 Diabetes Risk Genes in Human Pancreatic Islets. 2015 Nov 11. pii: db150956. [Epub ahead of print]
· Hardikar AA1,2, Satoor SN1, Karandikar MS1, Joglekar MV1, Puranik AS, Wong W, Kumar S, Limaye A, Bhat DS, Januszewski AS, Umrani MR, Ranjan AK, Apte K, Yajnik PC, Bhonde RR, Galande S, Keech AC, Jenkins AJ and Yajnik CS (2015) Susceptibility to obesity and diabetes following multigeneration undernutrition is not reversed by two generations of nutrient availability. Aug 4;22(2):312-9. doi: 10.1016/j.cmet.2015.06. 008. (Cover story) - Epub 2015 Jul 9. (1: Equal first author; 2: Corresponding author)
Farr RJ, Januszewski AS, Joglekar MV,Liang H, McAulley AK, Hewitt AW, Thomas HE, Loudovaris T, Kay TWH, Jenkins A, Hardikar A (2015) A comparative analysis of high-throughput platforms for validation of a circulating microRNA signature in diabetic retinopathy.
· Wong W, Farr RJ, Joglekar MV, Januszewski AS, Hardikar AA (in press) Probe-based real-time PCR approaches for quantitative measurement of microRNAs. J Visual Exp (accepted November 2014)
· Liu J, Joglekar MV, Sumer H, Hardikar AA, Teede H, Verma PJ (2014) Integration-Free Human Induced Pluripotent Stem Cells from Type 1 Diabetes Patient Skin Fibroblasts Show Upregulation of Pancreas-Specific microRNAs, : 7(1) 15-24
· Hardikar AA, Farr RJ, Joglekar MV (2014) Circulating microRNAs: Understanding the limits for quantitative measurement by real-time PCR 26;3(1):e000792. doi: 10.1161/JAHA.113.000792
Williams M, Joglekar MV, Ranjan AK, Rixon A and Hardikar AA (2014) Pdx1 (GFP/w) mice for isolation, characterization, and differentiation of pancreatic progenitor cells.
· Satoor SN, Patil DP, Kristensen HD, Joglekar MV, Shouche Y, Hardikar AA. (2014) Manipulation and assessment of gut microbiome for metabolic studies.
· Farr RJ, Joglekar MV, Taylor CJ, Hardikar AA. (2013) Circulating non-coding RNAs as biomarkers of beta cell death in diabetes. Pediatr Endocrinol Rev. 11 (1):14-20.
· Sundrani DP, Reddy US, Joshi AA, Mehendale SS, Chavan-Gautam PM, Hardikar AA, Chandak GR, Joshi SR (2013) Differential placental methylation and expression of VEGF, FLT-1 and KDR genes in human term and preterm preeclampsia. . 26;5(1):6. doi: 10.1186/1868-7083-5-6.
· Satoor SN and Hardikar AA (2013) Maternal nutrition, nutrient transfer & foetal pancreas development. .;137(2):249-50.
· Taylor CJ, Satoor SN, Ranjan AK, Pereira e Cotta MV and Joglekar MV (2012) A protocol for measurement of non-coding RNA in human serum. Exp Diabetes Res 2012:168368 (special issue on “ncRNAs”).
· Hardikar AA, Walker MD, Lynn F (2012) Non-codimng RNAs. Exp Diabetes Res. 629249. doi: 10.1155/2012/629249.
· Ranjan AK, Joglekar MV, Atre AN, Patole M, Bhonde RR and Hardikar AA (2012) Simultaneous Imaging of microRNA / mRNA Territories with Protein Territory in Mammalian Cells at Single Cell Resolution. RNA Biology 9:7, 949-53. doi: 10.4161/rna.20484
· Ranjan AK, Joglekar MV, Atre AN, Patole M, Bhonde RR and Hardikar AA (2012) Cellular detection of multiple antigens at single cell resolution using antibodies generated from the same species. J Imm Methods 379(1-2):42-7. doi: 10.1016/j.jim.2012.02.018. PMID: 22414487
· Joglekar MV and Hardikar AA (2012) Isolation, characterization and expansion of islet-derived progenitor cells. -66. doi: 10.1007/978-1-61779-815-3_21.
· Kumar U, Ranjan AK, Chandrashekhar S, Hardikar AA, Pundle A and Poddar P. (2012) Green approach towards size controlled synthesis of biocompatible antibacterial metal nanoparticles in aqueous phase using lysozyme. Curr Nanoscience 8(1), 130-140(11)
· Ansarullah, Bharucha B, Umarani M, Dwivedi M, Laddha NC, Begum R, Hardikar AA, Ramachandran AV. (2012) Oreocnide integrifolia Flavonoids Augment Reprogramming for Islet Neogenesis and β-Cell Regeneration in Pancreatectomized BALB/c Mice. Evid Based Comp Alt Med 2012-260467. PMID: 22474495
· Satoor SN, Puranik AS, Kumar S, Williams MD, Ghale M, Rahalkar A, Karandikar MS, Shouche Y, Patole M, Bhonde RR, Yajnik CS and Hardikar AA (2011) Location, location, location: Beneficial effects of autologous fat transplantation. Accepted for publication in Scientific Reports
· Vaithilingam V, Quayum N, Joglekar MV, Jensen J, Hardikar AA, Oberholzer J, Guillemin GJ, Tuch BE (2011) Effect of alginate encapsulation on the cellular transcriptome of human islets, Accepted for publication in Biomaterials
· Kumar U, Ranjan AK, Chandrashekhar S, Hardikar AA, Pundle A and Poddar P. (2011) Green approach towards size controlled synthesis of biocompatible antibacterial metal nanoparticles in aqueous phase using lysozyme. Accepted for publication in Current Nanoscience.
· Forster N, Penington A, Hardikar AA, Palmer J, Hussey A, Taj J, Morrison W, Feeney S (2011) A prevascularised tissue engineering chamber supports growth and function of islets and progenitor cells in diabetic mice. Islets (In press)
· Parekh VS, Umrani MR and Hardikar AA (2011) Taurine supports preservation of pro-endocrine cell types in human umbilical cord blood-derived mononuclear cells during cryostorage. (In press)
· Phadnis SM, Joglekar MV, Dalvi1 MP, Muthyala S, Nair PD, Ghaskadbi SM, Bhonde RR and Hardikar AA (2010) Human bone marrow-derived mesenchymal cells differentiate and mature into endocrine pancreatic lineage in vivo. (in press) Cover story
· Ansarullah, Jayaraman S, Hardikar AA and Ramachandran AV (2010) Influence of Oreocnide integrifolia (Gaud.) Miq on IRS-1, Akt and Glut-4 in Fat-Fed C57BL/6J Type 2 Diabetes Mouse Model. (In press).
· Joglekar MV, Wei CJ and Hardikar AA (2010) Quantitative estimation of multiple microRNAs and mRNAs from a single cell. Cold Spring Harb. Protocols Cover story
· Khandwekar AP, Patil DP, Hardikar AA, Shouche YS, Doble M. (2010) In vivo modulation of foreign body response on polyurethane by surface entrapment technique. J Biomed Mater Res A. (In press)
· Wu Y, Li J, Saleem S, Yee S, Hardikar AA and Wang R (2010) C-Kit and stem cell factor regulate PANC-1 cell differentiation to insulin- and glucagon-producing cells. (In press)
· Sasikala M, Swapna G, Pavan K, Rao GV, Hardikar AA and Reddy DN (2010) Reduced expression of pdx-1 correlates with decreased beta cell function in chronic pancreatitis. (In press)
· Lee J, Jo J, Hardikar AA, Periwal V and Rane SG (2010) Cdk4 regulates recruitment of quiescent β-cells and ductal epithelial progenitors to reconstitute β-cell mass PLoS ONE 13;5(1):e8653
· Phadnis SM, Ghaskadbi SM, Hardikar AA, Bhonde RR (2009) Mesenchymal stem cells derived from bone marrow of diabetic patients portrait unique markers influenced by the diabetic microenvironment. Rev Diabet Stud. 6(4):260-70.
· Ranjan AK, Kumar U, Hardikar AA, Poddar P, Nair PD, Hardikar AA (2009) Human blood vessel-derived endothelial progenitors for endothelialization of small diameter vascular prosthesis. PLoS ONE 4(11):e7718.PMID: 19890388
· Joglekar MV, Patil D, Joglekar VM, Rao GV, Reddy DN, Sasikala M, Shouche Y and Hardikar AA (2009) The miR-30 Family microRNAs Confer Epithelial Phenotype to Human Pancreatic Cells. 1(2): 137 - 147. Cover story
· Parekh VS, Joglekar MV and Hardikar AA (2009) Differentiation of human umbilical cord blood derived mononuclear cells to endocrine pancreatic lineage. 78(4):232-40. Cover story
· Joglekar MV, Joglekar VM, Joglekar SV and Hardikar AA (2009), Human fetal pancreatic insulin-producing cells proliferate in vitro. 201(1):27-36, Epub 2009 May 6 Cover story
· Sahu S*, Joglekar MV*, Dumbre R, Phadnis SM, Tosh D and Hardikar AA, (2009) Islet-like cell clusters occur naturally in human gall bladder and are retained in diabetic conditions : 13(5):999-1000; *: equal first authors.
· Joglekar MV, Joglekar VM and Hardikar AA (2009) Expression of islet-specific microRNAs during human pancreatic development. Gene Exp Patterns; 9(2): 109-113. Epub 2008 Oct 17
· Joglekar MV, Parekh VS, Mehta S, Bhonde RR and Hardikar AA (2007) MicroRNA Profiling of Developing and Regenerating Pancreas Reveal Post-transcriptional Regulation of Neurogenin3. Developmental Biology 311(2): 603-612
· Phadnis SM*, Joglekar MV*, Venkateshan V, Ghaskadbi SM, Hardikar AA, Bhonde RR. (2006) Human umbilical cord blood serum promotes growth, proliferation, as well as differentiation of human bone marrow-derived progenitor cells. In Vitro Cell Dev Biol Anim. 42(10):283-6.
· Gershengorn MC, Geras-Raaka E, Hardikar AA, Raaka BM. (2005) Are better islet cell precursors generated by epithelial-to-mesenchymal transition? Cell Cycle. 4:380-382.
· Gershengorn MC, Hardikar AA, Wei C, Geras-Raaka E, Marcus-Samuels B, Raaka BM (2004) Epithelial-to-Mesenchymal Transition Generates Proliferative Human Islet Precursor Cells. Science 306:2261-2264.
· Hardikar AA, Marcus-Samuels B, Geras-Raaka E, Raaka BM, Gershengorn MC (2003) Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates. Proc Natl Acad Sci U S A. 100(12): 7117-7122.
· Hardikar AA, Wang XY, Williams LJ, Kwok J, Wong R, Yao M, Tuch BE. (2002) Functional maturation of fetal porcine beta-cells by glucagon-like peptide 1 and cholecystokinin. Endocrinology; 143:3505-3514.
· Merezek S, Hardikar AA, Yajnik CS, Remacle C and Reusens B (2001) Intra-uterine protein malnutrition and diabetes: the role of sulphur amino acids. J Endocrinol; 171:299-308.
· Hardikar AA, Risbud MV, Remacle C, Reusens B, Hoet JJ, Bhonde RR. (2001) Islet cryopreservation: improved recovery following taurine pretreatment. Cell Transplant; 10:247-253.
· Hardikar AA, Nath BB. (2001) Chromosomal polymorphism is associated with nematode parasitism in a natural population of a tropical midge. Chromosoma; 110: 58-64.
· Risbud MV, Hardikar AA, Bhat SV, Bhonde RR. (2000) pH-sensitive freeze-dried chitosan-polyvinyl pyrrolidone hydrogels as controlled release system for antibiotic delivery. J Control Release; 68: 23-30.
· Hardikar AA, Risbud MV, Bhonde RR. (2000) Improved post-cryopreservation recovery following encapsulation of islets in chitosan-alginate microcapsules. Transplant Proc; 32: 824-5.
· Risbud M, Hardikar A, Bhonde R. (2000) Growth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management? J Biosci ; 25: 25-31.
· Risbud M, Hardikar A, Bhonde R. (2000) Chitosan-polyvinyl pyrrolidone hydrogels as candidate for islet immunoisolation: in vitro biocompatibility evaluation. Cell Transplant; 9 : 25-31.
· Hardikar AA, Bhonde RR. (1999) Modulating experimental diabetes by treatment with cytosolic extract from the regenerating pancreas. Diabetes Res Clin Pract; 46: 203-11.
· Hardikar AA, Karandikar MS, Bhonde RR. (1999) Effect of partial pancreatectomy on diabetic status in BALB/c mice. J Endocrinol ; 162: 189-195.
· Hardikar AA, Risbud MV and Bhonde RR (1999) A simple microcapsule generator design for islet encapsulation, J. Biosci. 24 (3): 371 - 376.
· Ranjan AK, Joglekar MV and Hardikar AA (2014) Characterizing Cellular Identity at One Cell Resolution in In Situ Hybridization Methods eds Giselbert Hauptmann, Springer Press, NY, USA
· Wong W, Joglekar MV, Satoor SN, Sahu S, Parekh VS and Hardikar AA (2014) Lineage-committed Pancreatic Progenitors and Stem Cells. In Adult Stem Cells eds Kursad Turksen, Springer Press, NY, USA
· Sahu S, Joglekar M, Yang S and Hardikar AA (2011) Gallbladder-derived islet stem cells, In "Stem Cells", ISBN: 978-953-307-412-2. Open Access Publishers, Vienna, Austria.
· Lie D, Chayosumrit M, Hardikar A and Sidhu K (2010) Puluripotent stem cells and diabetes: Basic Science to Clinical Applications in: Frontiers in pluripotent stem cells research: Bench-to-Bedside Publisher: Betham Press, Editor: K Sidhu
Joglekar MV and Hardikar AA (2010) Human pancreatic progenitors:
implications for clinical transplantation in Diabetes in “Human Fetal
Tissue in Transplantation” eds. Dr Niranjan
Bhattacharya and Prof Phillip Stubblefield. Springer Verlag,
· Hardikar AA and Gershengorn MC (2003) Chapter 51: ‘Stem Cells’ in 3rd edition of ‘Diabetes Mellitus: A Fundamental and Clinical Text’, edited by: Dr. Derek LeRoith, Simeon I. Taylor and Jerrold M. Olefsky.
· Shukla R, Risbud MR, Hardikar AA, Bhonde RR, (2004) Chitosan and chitin derivatives in islet immunoisolation and transplantation. In “Chitin and Chitosan: Opportunities and Challenges” Chapter 15, p 211-222, eds; Dutta PK
· Joglekar MV, Parekh VS and Hardikar AA (2011) microRNAs in endocrine pancreas development, differentiation and disease. In J Exp. Biol 49(6):401-8.
· Williams MD, Mitchell GM and Hardikar AA, Stem cells: Epigenetic basis of differentiation (2010) (in press)
· Dalvi MP, Umrani MR, Joglekar MV, Hardikar AA (2009) Human pancreatic islet progenitor cells demonstrate phenotypic plasticity in vitro. J Biosci. 34(4):523-8.
· Kode JA, Mukherjee S, Joglekar MV and Hardikar AA (2009) Mesenchymal Stem cells: Immunobiology and role in immunomodulation and tissue regeneration. : 11(4):377-91
· Joglekar MV, Parekh VS and Hardikar AA (2007) New Pancreas from Old: Microregulators of Pancreas Regeneration Trends Endocrinol Metab 18(10):393-400. Cover citation.
AA, Lees JG,
· Hardikar AA (2004) Pancreas regeneration: Generating new pancreas from old. Trends Endocrinol Metab. 15:198-203 Cover story
· Joglekar MV and Hardikar AA (2010) Epithelial-to-mesenchymal transition in pancreatic islet beta cells. Cell Cycle (in press)
· Hardikar AA and Tosh D (2009) Commentary: β-cells in the lab. Nature India, doi 10:1038/nindia.2009.160
· Sahu S, Tosh D and Hardikar AA (2009) Commentary: New sources of beta-cells for treating diabetes. J. Endocrinology: 202(1):13-6
· Hardikar A, (2004) Role of Incretins in Pancreas Growth and Development. J Pancreas 5(6):454-456.
· The RAPID Study: RNA-based Analysis for Prediction of Islet Death- mRNA and non-codingRNAs as biomarkers of diabetes and complications
· Understanding the role of ncRNAs in pancreatic development, differentiation and disease
· Role of micronutrients and gut microbiota in development of central adiposity and diabetes
· Differentiation of human gallbladder-derived endocrine progenitor cells
· Epigenetic mechanisms regulating beta cell function
Research facilities in the Hardikar laboratory include a human cell culture facility, rodent cell culture facility, human and mouse ES cell culture facility, real-time pcr (Applied Biosystems ViiA 7, 7500 FAST, 7900 HT FAST block and TLDA systems), Calcium ratio imaging system (Till Photonics), Axio Observer Z1 with Apotome imaging, PALM microbeam 4 laser catapulting system, in vivo imaging facility and the Ion Torrent personal genome sequencer. Other accessible central facilities include proteomics and microarray core, flowcytometry core (BD FACS Vantage, MoFlow, Aria, Caliburs, Canto II) and imaging facility (LSM 510, TIRF, FCS, time-lapse microscopy), DNA sequencing facility and high throughput sequencing (Applied Biosystems SOLiD).
Research in the Hardikar laboratory (in India) has been funded by intramural funding from the National Center for Cell Science and research project grants from the Department of Biotechnology, Government of India and the UK-India Educational Research Initiative (UKIERI) / British Council, UK. Research work at O’Brien Institute was supported through generous funding from Microsurgery Foundation and The Barbara Walker Fellowship. Research at the University of Sydney is supported through national competitive grants from the National Health and Medical Research Council (NHMRC) and the Australian Research Council (ARC) and other funding agencies including the Diabetes Australia Research Trust, the Weizmann-Australia Initiative and the Juvenile Diabetes Research Foundation. Infrastructure support to the Hardikar lab from above funding agencies and specifically from the Rebecca L. Cooper Foundation is kindly acknowledged.
The research group is based at the NHMRC Clinical Trials Centre in Medical Foundation Building at the University of Sydney. Potential students interested in research can e-mail their CV along with names and contact information of 3 referees. International students can access fellowship possibilities for PhD programs by visiting the University of Sydney “Future students” website.
For more information about existing and past lab members from the Hardikar lab, please click here
PhD fellowships: The Hardikar lab currently has position for 1 Honor’s students to be accommodated at the University of Sydney. Applications are invited from Australian citizens or permanent residents of Australia. Please enclose a CV with details of WAM scores, work experience and a statement of purpose. Please e-mail all applications to A/Prof. Hardikar for further consideration.
Professor Anthony C. Keech, NHMRC Clinical Trials Centre, University of Sydney, Australia
Professor Alicia Jenkins, NHMRC Clinical Trials Centre, University of Sydney, Sydney, Australia
Thomas Kay and A/Professor Helen Thomas, St. Vincent’s Institute,
Alex Hewitt, Center for Eye Research
Dr. David Martin, Laparoscopic, General and Obesity Surgeon, Sydney, Australia
Professor Shimon Efrat, Tel Aviv University, Israel
Dr. Paul Verma, Monash Institute of Medical Research,
Professor Ed Stanley, Monash Immunology and Stem Cells Laboratories, Clayton, Australia
Dr. David Tosh,
Professor Sanjeev Galande, Indian Institute of Science and Education Research, Pune
Sushil G. Rane,
National Institutes of Health,
Dr. DN Reddy and Dr. GV Rao, Asian Institute of Gastroenterology, India
Dr. Yogesh Shouche, Microbial
Len Harrison, WEHI,
Dhan Thiruchelvam, St.
Dr. Vinay and Dr. Sheela Joglekar, Shree Seva Medical Foundation, Shirwal
Dr. Medha Patankar, Patankar Nursing Home, Pune
The Diabetes and Islet-biology group is located on Level 1 of the Medical Foundation Building, 92-94 Parramatta Road, The University of Sydney, Camperdown, NSW 2050.
Last revised: January 11, 2014