Clinical good manufacturing practice (GMP)-based human islet isolation is a cost- and resource-intensive program wherein cadaveric human islets are made available for transplantation to treat individuals with Type 1 diabetes (T1D). The Collaborative Islet Transplant Registry (CITR) data indicate that donor characteristics alone are not good predictors of islet yield, viability, and function. There is a major unmet need to identify biomarker(s) that efficiently predict the quality of islets before initiating their isolation from the cadaveric pancreas. We recently demonstrated (Wong WKM et al JCI insight 2019) the capacity of two variants of a long-non-codingRNA (called NEAT2 a.k.a MALAT1) to predict islet quality prior to their isolation. The planned mouse studies will provide means to understand mechanisms that help enhance the stratification of donor pancreas to GMP-grade; for clinical islet transplantation , whilst redirecting the other (non-GMP grade) donor pancreas to research-grade islet isolation workflows. Congratulations Wilson on this award!!
Congratulations to Dr. Barbora Paldus ( from SVH, Melbourne), who was also awarded the 2020 ADS Lindsey Baudinet Award this year! Great to see two awards during such a tough year and grateful to the Australian Diabetes Society and the Lindsey Baudinet family for making this possible! Thank you!!
Video can’t be displayed
This video is not available.
MALAT1 lncRNA expression in human islets
Our work on understanding the predictive power of MALAT1 lncRNA measurement in human pancreas is now online in JCI Insight. This study demonstrates our analysis wherein a whole transcriptome (discovery) analyses using next generation sequencing platform and machine learning workflow identified gene variants of the lncRNA MALAT1 to be highly expressed in Good quality islets. We validated these NGS data by PCR in 75 more human islet preparations. Then in a new set of 19 human pancreas samples, we show that measuring the expression levels of MALAT1 variants can predict the post-isolation islet quality. Please watch this video prepared to provide an introduction to this work.
Through the NHMRC project grant scheme, we are funded to validate a panel of RNA-based biomarkers that were identified by our group to be associated with endothelial damage in diabetes. A one year Research Assistant position has been advertised. Email us for any further details or follow this link to read more about the job description.
PREDICT T1D Study video
American Diabetes Association (2018), Orlando, USA
This video provides information related to the research on validating miRNA (and insulin cfDNA) biomarkers of Type 1 diabetes in over 2000 plasma samples from individuals without, with or at risk of Typ[e 1 diabetes. The video was generated through an invitation from the American Diabetes Association (ADA TV) and generated through support from ThermoFisher Scientific. Research presented through this video has mainly been funded through the Australian Future Fellowship (2012-16) from the Australian Research Council, a pilot & Feasibility grant from JDRF Australia, The T1D Clinical Research Network Career Development Award from JDRF Australia (2016-2020) and a JDRF Australia/Helmsley Trust innovation grant (2018-21) to A/Prof. Hardikar.
Research presentations in Denmark
DDA meeting on noncodingRNAs in metabolic disease
A/Prof Hardikar was invited to present T1D biomarker research at the ncRNA metabolic diseases meeting organized by the Danish Diabetes Academy(6-8 May 2019): Novo Nordisk Foundation, Tuborg Havnevej 19, 2900 Hellerup, Denmark.
Dr. Wilson Wong also presented our recent research findings on identifying lncRNA variants that predict the quality of human islets prior to their isolation from cadaveric human pancreas.
Vijit Saini, one of our PhD students, also presented a poster on our research related to understanding the role of microRNAs that are associated with insulin gene transcription. The Hardikar Lab acknowledges the generous support from JDRF Australia Travel Grants to support Dr. Wilson Wong and Mr. Vijit Saini's travel to Denmark.
About Our laboratory
The Hardikar Laboratory (Islet Biology and Diabetes Group) is located in the Ainsworth Building (Building 30) of the Western Sydney University, Campbelltown campus.
Parking is available in designated car spots across P1 and P2 parking lots next to the building or a short walk from the Macarthur train station.