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AI-based workflows

ML-based workflows
We validate our ML workflows across independent data scientists with pathways in different programming languages.
  • Assessing the best ML workflow
  • Comparing decision tree analyses (Ensemble tools) with simpler regression models by integrating bootstrapping.
  • Integration of image, clinical, geopositional or biomarker data
  • Interpretation with our own and existing datasets
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Generative AI
Generative AI offers the capacity to map a virtually unlimited number of synthetic individuals with biomarker profiles that follow the distribution of biomarkers in real-life samples. There are over 10 different synthetic data generation workflows, each with distinct libraries in R and Python - which one is best for you?
  • Synthetic clones
  • Augmented data in model training
  • Multi-context applicability and GAI workflow selection
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Predictive analysis
Utilising clinical, biochemical, molecular, socio-economic, geopositional and other relevant data to drive accurate prediction of future health.
  • Study design
  • Suitable AI/ML strategies
  • Use of our tissue/data bank
  • Predictive analyses and independent validation.
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Personalised medicine
Our AI-based workflows are designed to drive personalised decision making, cohort-based risk stratification as well as context (ethnicity/race)-driven predictive risk scores.
  • Context-driven predictions
  • Dynamic risk scores
  • Static risk score assessment
  • Integrated learning for personalised solutions to healthcare
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All codes for workflows used/published by our group are available through Github/isletbiology/ or through collaborative arrangements and support by contacting Professor Hardikar.
Related research articles/preprint from our group: 10) Applicability of a microRNA-based Dynamic Risk Score (DRS) for type 1 diabetes Hardikar HP, Thorat V, Kunte PS, Kulkarni RA, Pant A, Wong KMW, Joglekar MV and Hardikar AA, on behalf of the PREDICT T1D Study Group (2025) Code Ocean. https://doi.org/10.24433/CO.4476520.v1 This presents the source code used in our Nat Med paper. The base (10,000 synthetic individuals) model is presented here. Please get in touch with us if you are interested in using larger (>100k) and other (relevant to your dataset) synthetic data generation pipelines for achieving Generative AI-based enhancement to your predictive models. 9) A microRNA-based dynamic risk score for Type 1 Diabetes. Joglekar MV*, Wong WKM*, Kunte PS*, Hardikar HP*, Kulkarni RA, Ahmed I, Farr RJ, Pham NHT, Coles M, Kaur S, Maynard CL, Hayward R, Thorat V, Pant A, Akil AA, Donaghue K, Jenkins A, Piya MK, Craig ME, Hague W, Yajnik CS, Chan JCS, Shapiro AMJ, Davis EA, Jones TW, Gitelman SE, Ma RCW, Pociot F and Hardikar AA, on behalf of the PREDICT T1D Study Group° (2025) Nature Medicine. 025 Jun 5. doi: 10.1038/s41591-025-03730-7. Online ahead of print.. *: equal first author. MVJ &AAH are co-corresponding authors. PMID: 40473952 DOI: 10.1038/s41591-025-03730-7 8) Capturing dynamic rather than static risk for improved monitoring of type 1 diabetes progression This Nat Med briefing was compiled by Hardikar AA and Joglekar MV with the Nat Med editorial team and presents a more lay review of our original article (see above) published in the same issue (August 2025) of Nature Medicine.(2025) Nature Medicine PMID: 40571755. DOI: 10.1038/s41591-025-03825-1 7) Prediction of progression to type 1 diabetes with dynamic biomarkers and risk scores. Joglekar MV, Kaur S, Pociot F, Hardikar AA Lancet Diabetes Endocrinol. 2024 Jul;12(7):483-492. doi: 10.1016/S2213-8587(24)00103-7. Epub 2024 May 23.PMID: 38797187 6) Circulating microRNAs from early childhood and adolescence are associated with pre-diabetes at 18 years of age in women from the PMNS cohort.Joglekar MV, Kunte PS, Wong WKM, Bhat DS, Satoor SN, Patil RR, Karandikar MS, Fall CHD, Yajnik CS, Hardikar AA.J Dev Orig Health Dis. 2022 Dec;13(6):806-811. doi: 10.1017/S2040174422000137. Epub 2022 Apr 22.PMID: 35450554 5) Analysis of Half a Billion Datapoints Across Ten Machine-Learning Algorithms Identifies Key Elements Associated With Insulin Transcription in Human Pancreatic Islet Cells.Wong WKM, Thorat V, Joglekar MV, Dong CX, Lee H, Chew YV, Bhave A, Hawthorne WJ, Engin F, Pant A, Dalgaard LT, Bapat S, Hardikar AA.Front Endocrinol (Lausanne). 2022 Mar 23;13:853863. doi: 10.3389/fendo.2022.853863. eCollection 2022.PMID: 35399953 4) Manipulating cellular microRNAs and analyzing high-dimensional gene expression data using machine learning workflows.Saini V, Joglekar MV, Wong WKM, Jiang G, Nassif NT, Simpson AM, Ma RCW, Dalgaard LT, Hardikar AA.STAR Protoc. 2021 Oct 23;2(4):100910. doi: 10.1016/j.xpro.2021.100910. eCollection 2021 Dec 17.PMID: 34746868 3) Machine learning workflows identify a microRNA signature of insulin transcription in human tissues.Wong WKM, Joglekar MV, Saini V, Jiang G, Dong CX, Chaitarvornkit A, Maciag GJ, Gerace D, Farr RJ, Satoor SN, Sahu S, Sharangdhar T, Ahmed AS, Chew YV, Liuwantara D, Heng B, Lim CK, Hunter J, Januszewski AS, Sørensen AE, Akil ASA, Gamble JR, Loudovaris T, Kay TW, Thomas HE, O'Connell PJ, Guillemin GJ, Martin D, Simpson AM, Hawthorne WJ, Dalgaard LT, Ma RCW, Hardikar AA.iScience. 2021 Mar 31;24(4):102379. doi: 10.1016/j.isci.2021.102379. eCollection 2021 Apr 23.PMID: 33981968 2) Postpartum circulating microRNA enhances prediction of future type 2 diabetes in women with previous gestational diabetes.Joglekar MV, Wong WKM, Ema FK, Georgiou HM, Shub A, Hardikar AA, Lappas M.Diabetologia. 2021 Jul;64(7):1516-1526. doi: 10.1007/s00125-021-05429-z. Epub 2021 Mar 23.PMID: 33755745 . AAH: Corresponding author who led the submission and is co-corresponding with ML who provided study samples and related metadata to MVJ and AAH for analysis. 1) The long noncoding RNA MALAT1 predicts human pancreatic islet isolation quality.Wong WK, Jiang G, Sørensen AE, Chew YV, Lee-Maynard C, Liuwantara D, Williams L, O'Connell PJ, Dalgaard LT, Ma RC, Hawthorne WJ, Joglekar MV, Hardikar AA.JCI Insight. 2019 Jul 30;5(16):e129299. doi: 10.1172/jci.insight.129299.PMID: 31361602

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